rabies virus tracing Search Results


penn viral vector core antibody anti wild type rabies phosphoprotein mouse monoclonal antibody  (Vector Laboratories)
95
Vector Laboratories penn viral vector core antibody anti wild type rabies phosphoprotein mouse monoclonal antibody
Figure 1. Trans-synaptic <t>wild-type</t> rabies tracing reveals both closed and open cortico-basal ganglia loops. (A) The strategy to label striatal neurons connecting to the cerebral cortex by Wt-RABV trans-synaptic retrograde tracing, and CTb-based non-trans-synaptic anterograde tracing for mapping cortico-striatal terminals. (B) Example image of Wt-RABV/CTb injection into M1 (left). After 66–70 hr of survival time, Wt-RABV was transfected up to 3rd- order neurons, which were found in various striatal subregions (right). Scale bars, 1 mm (left), 500 mm (right). (C) 3D-reconstruction of Wt-RABV+ striatal neurons from the M1 injection case shown in (B). The two different angles emphasize the presence of Wt-RABV+ neurons throughout all of the striatum (VS, DMS, DLS, and TS). (D) Schema of Wt-RABV/CTb injection in M1. (E) Anterogradely labeled CTb+ cortico-striatal terminals (green) and retrogradely labeled Wt-RABV+ striatal neurons (purple) from the M1 injection case shown in (B). (F) Density map showing the distribution of Wt-RABV+ neurons throughout the striatum from M1 injection. Black contours indicate approximate areas receiving cortico-striatal inputs from M1. Color maps indicate the intensity of Wt-RABV+ labeling. (G–I) The same analyses for Wt-RABV/CTb injection in M2. (J–L) The same analyses for Wt-RABV/CTb injection in mPFC. (M) Normalized distribution of Wt-RABV+ neurons across five striatal regions (VMS, VLS, DMS, DLS, TS) showing differences Figure 1 continued on next page
Penn Viral Vector Core Antibody Anti Wild Type Rabies Phosphoprotein Mouse Monoclonal Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabies+virus+tracing/10__7554_slash_elife__49995-240-92-94?v=Vector+Laboratories
Average 95 stars, based on 1 article reviews
penn viral vector core antibody anti wild type rabies phosphoprotein mouse monoclonal antibody - by Bioz Stars, 2026-08
95/100 stars
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rabies virus tracing  (Addgene inc)
93
Addgene inc rabies virus tracing
Figure 1. Trans-synaptic <t>wild-type</t> rabies tracing reveals both closed and open cortico-basal ganglia loops. (A) The strategy to label striatal neurons connecting to the cerebral cortex by Wt-RABV trans-synaptic retrograde tracing, and CTb-based non-trans-synaptic anterograde tracing for mapping cortico-striatal terminals. (B) Example image of Wt-RABV/CTb injection into M1 (left). After 66–70 hr of survival time, Wt-RABV was transfected up to 3rd- order neurons, which were found in various striatal subregions (right). Scale bars, 1 mm (left), 500 mm (right). (C) 3D-reconstruction of Wt-RABV+ striatal neurons from the M1 injection case shown in (B). The two different angles emphasize the presence of Wt-RABV+ neurons throughout all of the striatum (VS, DMS, DLS, and TS). (D) Schema of Wt-RABV/CTb injection in M1. (E) Anterogradely labeled CTb+ cortico-striatal terminals (green) and retrogradely labeled Wt-RABV+ striatal neurons (purple) from the M1 injection case shown in (B). (F) Density map showing the distribution of Wt-RABV+ neurons throughout the striatum from M1 injection. Black contours indicate approximate areas receiving cortico-striatal inputs from M1. Color maps indicate the intensity of Wt-RABV+ labeling. (G–I) The same analyses for Wt-RABV/CTb injection in M2. (J–L) The same analyses for Wt-RABV/CTb injection in mPFC. (M) Normalized distribution of Wt-RABV+ neurons across five striatal regions (VMS, VLS, DMS, DLS, TS) showing differences Figure 1 continued on next page
Rabies Virus Tracing, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabies+virus+tracing/pmc09980144-280-0-5?v=Addgene+inc
Average 93 stars, based on 1 article reviews
rabies virus tracing - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier

Image Search Results


Figure 1. Trans-synaptic wild-type rabies tracing reveals both closed and open cortico-basal ganglia loops. (A) The strategy to label striatal neurons connecting to the cerebral cortex by Wt-RABV trans-synaptic retrograde tracing, and CTb-based non-trans-synaptic anterograde tracing for mapping cortico-striatal terminals. (B) Example image of Wt-RABV/CTb injection into M1 (left). After 66–70 hr of survival time, Wt-RABV was transfected up to 3rd- order neurons, which were found in various striatal subregions (right). Scale bars, 1 mm (left), 500 mm (right). (C) 3D-reconstruction of Wt-RABV+ striatal neurons from the M1 injection case shown in (B). The two different angles emphasize the presence of Wt-RABV+ neurons throughout all of the striatum (VS, DMS, DLS, and TS). (D) Schema of Wt-RABV/CTb injection in M1. (E) Anterogradely labeled CTb+ cortico-striatal terminals (green) and retrogradely labeled Wt-RABV+ striatal neurons (purple) from the M1 injection case shown in (B). (F) Density map showing the distribution of Wt-RABV+ neurons throughout the striatum from M1 injection. Black contours indicate approximate areas receiving cortico-striatal inputs from M1. Color maps indicate the intensity of Wt-RABV+ labeling. (G–I) The same analyses for Wt-RABV/CTb injection in M2. (J–L) The same analyses for Wt-RABV/CTb injection in mPFC. (M) Normalized distribution of Wt-RABV+ neurons across five striatal regions (VMS, VLS, DMS, DLS, TS) showing differences Figure 1 continued on next page

Journal: eLife

Article Title: An open cortico-basal ganglia loop allows limbic control over motor output via the nigrothalamic pathway

doi: 10.7554/elife.49995

Figure Lengend Snippet: Figure 1. Trans-synaptic wild-type rabies tracing reveals both closed and open cortico-basal ganglia loops. (A) The strategy to label striatal neurons connecting to the cerebral cortex by Wt-RABV trans-synaptic retrograde tracing, and CTb-based non-trans-synaptic anterograde tracing for mapping cortico-striatal terminals. (B) Example image of Wt-RABV/CTb injection into M1 (left). After 66–70 hr of survival time, Wt-RABV was transfected up to 3rd- order neurons, which were found in various striatal subregions (right). Scale bars, 1 mm (left), 500 mm (right). (C) 3D-reconstruction of Wt-RABV+ striatal neurons from the M1 injection case shown in (B). The two different angles emphasize the presence of Wt-RABV+ neurons throughout all of the striatum (VS, DMS, DLS, and TS). (D) Schema of Wt-RABV/CTb injection in M1. (E) Anterogradely labeled CTb+ cortico-striatal terminals (green) and retrogradely labeled Wt-RABV+ striatal neurons (purple) from the M1 injection case shown in (B). (F) Density map showing the distribution of Wt-RABV+ neurons throughout the striatum from M1 injection. Black contours indicate approximate areas receiving cortico-striatal inputs from M1. Color maps indicate the intensity of Wt-RABV+ labeling. (G–I) The same analyses for Wt-RABV/CTb injection in M2. (J–L) The same analyses for Wt-RABV/CTb injection in mPFC. (M) Normalized distribution of Wt-RABV+ neurons across five striatal regions (VMS, VLS, DMS, DLS, TS) showing differences Figure 1 continued on next page

Article Snippet: DOI: https://doi.org/10.7554/eLife.49995 15 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (recombinant rabies virus) EnvA.dGRabies.eGFP Salk Vector Core RRID: SCR_014847 Strain, strain background (adeno-associated virus) AAV9.FLEX.tdTomato University of Penn Viral Vector Core RRID: SCR_015406 Strain, strain background (adeno-associated virus) AAV9.FLEX.eGFP University of Penn Viral Vector Core RRID: SCR_015406 Strain, strain background (adeno-associated virus) AAV9.CAG.tdTomato UNC Viral Vector Core RRID: SCR_002448 Strain, strain background (adeno-associated virus) AAV9.hsyn.ChR2.eGFP University of Penn Viral Vector Core RRID: SCR_015406 Strain, strain background (adeno-associated virus) AAV5-EF1a-DIOhChR2(H134R)-mCherry University of Penn Viral Vector Core Antibody anti-wild type rabies phosphoprotein mouse monoclonal antibody commercially unavailable (Raux et al., 1997) (1:5000) Antibody anti-cholera toxin b-subunit goat polyclonal antibody List Biological Laboratories Cat.# 704 (1:15000) Antibody anti-tyrosine hydroxylase (TH) mouse monoclonal antibody Millipore Cat.# MAB318 (1:1000) Antibody anti-NeuN rabbit polyclonal antibody Abcam Cat.# ab104225 (1:1000) Antibody anti-GFP chicken polyclonal antibody Novus Biologicals Cat.# NB100-1614 (1:1000) Antibody anti-substance P mouse monoclonal antibody Abcam Cat.# ab14184 (1:1000) Antibody anti-mouse IgG horseradish peroxydase (HRP) (host: rabbit, polyclonal) DAKO Cat.# P260 (1:200) Antibody anti-goat IgG horseradish peroxydase (HRP) (host: rabbit, polyclonal) DAKO Cat.# P044901-2 (1:200) Antibody anti-mouse Alexa Fluor 488 (host: donkey, polyclonal) Jackson ImmunoResearch Laboratories Cat.# 715-545-151 (1:250) Antibody anti-mouse Cy3 (host: donkey, polyclonal) Jackson ImmunoReseach Laboratories Cat.# 715-165-151 (1:250) Antibody anti-mouse Cy5 (host: donkey, polyclonal) Jackson ImmunoReseach Laboratories Cat.# 715-175-151 (1:250) Continued on next page Aoki et al. eLife 2019;8:e49995.

Techniques: Retrograde Tracing, Anterograde Tracing, Injection, Transfection, Labeling